genome-alignment-qc
Assess alignment quality with coverage depth, mapping rates, insert size, and on-target metrics using samtools, mosdepth, and picard.
Browse curated skills with source links, package snapshots, README assets and install signals in one calm, searchable catalog.
Assess alignment quality with coverage depth, mapping rates, insert size, and on-target metrics using samtools, mosdepth, and picard.
Call SNVs and indels from aligned BAMs using GATK HaplotypeCaller or DeepVariant with optional GVCF output for joint genotyping.
Writes Specific Aims pages for grant applications. Use when drafting or revising the Specific Aims page (NIH R01/R21/R03), NSF Project Summary, or equivalent for any major funding agency. Also trigger…
Expert guidance for GRPO/RL fine-tuning with TRL for reasoning and task-specific model training
Parameter-efficient fine-tuning for LLMs using LoRA, QLoRA, and 25+ methods. Use when fine-tuning large models (7B-70B) with limited GPU memory, when you need to train <1% of parameters with minimal a…
Build a traceability manifest linking analysis scripts to inputs, outputs, and manuscript figures or tables. Use when documenting a computational pipeline or locating the source of a reported artefact…
Find full-text download links for a PubMed paper by PMID - DOI, PMC open access, Sci-Hub, and publisher links. Use when user wants to read or download a paper's full text.
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Align short reads to a reference genome with BWA-MEM2, sort and index with samtools, and mark duplicates with picard.
Filter raw variant calls using GATK VQSR, GATK hard filters, or bcftools expression-based filtering.
Drafting and reviewing European fellowship and grant applications (Marie Skłodowska-Curie Actions, Horizon Europe, ERC, EIC). Covers the Excellence/Impact/Implementation triad, gender dimension and se…
在本机检查 DOCX、PPTX、XLSX 和 PDF 交付材料中的金额、百分比、日期、单位及同比环比冲突,并输出带文件位置的易读报告。用户要求跨文档核对、交付前质检、检查 PPT 与 Excel 或保护敏感文档时使用。
Build and analyze phylogenetic trees using MAFFT (multiple alignment), IQ-TREE 2 (maximum likelihood), and FastTree (fast NJ/ML). Visualize with ETE3 or FigTree. For evolutionary analysis, microbial g…
Perform advanced PubMed search with field qualifiers - author, title, journal, MeSH, date range, article type. Constructs proper PubMed query syntax from natural language. Use for precise filtered sea…
Navigate PubMed search result pages or change sort order. Use when user wants to see more results or change ordering.
analyze a professor from google scholar, publication lists, personal websites, lab pages, and field-specific bibliographic databases (e.g., DBLP, PubMed, SSRN, PhilPapers, MathSciNet, arXiv, Scopus) t…
Annotate variants with functional effects using SnpEff or Ensembl VEP, adding gene impact, population frequencies, and clinical significance.
Comprehensive geospatial science skill covering remote sensing, GIS, spatial analysis, machine learning for earth observation, and 30+ scientific domains. Supports satellite imagery processing (Sentin…
Converts a biomedical study storyline into a graphical abstract and, when direct image capability is available, generates the graphical abstract directly; otherwise it falls back to prompts, Mermaid f…
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Export PubMed paper(s) to Zotero or save as RIS file. Supports single paper by PMID or batch export from search results. Use when user wants to save papers to their reference manager.
High-performance genomic interval operations and bioinformatics file I/O on Polars DataFrames. Overlap, nearest, merge, coverage, complement, subtract for BED/VCF/BAM/GFF intervals. Streaming, cloud-n…
Use this skill whenever a user wants to adopt the scicomp-research-skills framework on an EXISTING project (paper, software library, analysis repo) rather than starting from scratch -- OR when the age…