HUMAnN Functional Profiler Skill

Purpose

Provide HUMAnN functional profiling for metagenomic pathway analysis.

Capabilities

• Gene family quantification
• MetaCyc pathway abundance
• UniRef annotation
• Stratified functional profiles
• Pathway coverage analysis
• Custom database integration

Usage Guidelines

• Run taxonomic profiling first
• Quantify gene families and pathways
• Stratify by organism when relevant
• Analyze pathway coverage
• Compare functional profiles
• Document database versions

Dependencies

• HUMAnN3
• eggNOG-mapper
• Prokka

Process Integration

• Shotgun Metagenomics Pipeline (shotgun-metagenomics)