QIIME2 Microbiome Analyzer Skill

Purpose

Enable QIIME2 microbiome analysis for 16S rRNA profiling and diversity analysis.

Capabilities

• Demultiplexing and denoising (DADA2)
• Taxonomic classification
• Alpha diversity metrics
• Beta diversity analysis
• Differential abundance testing
• Phylogenetic analysis

Usage Guidelines

• Use DADA2 for denoising amplicon data
• Select appropriate taxonomic classifier
• Calculate diversity metrics with rarefaction
• Visualize beta diversity with ordination
• Test differential abundance appropriately
• Document pipeline parameters

Dependencies

• QIIME2
• DADA2
• phyloseq

Process Integration

• 16S rRNA Microbiome Analysis (16s-microbiome-analysis)